PHF1 and Alzheimer disease: 6-month-old 3xTg-AD animals injected with shSCRmiR and sacrificed 1 year later showed a PHF1 immunoreactive band in 65 kDa (pSer-396/404; Greenberg and Davies, 1990; Kimura et al., 2010), characteristic of hyperphosphorylated Tau in the soluble fraction and in sarkosyl-insoluble fraction (Figure 2A).