ACTA1 and neoplasm: Using this method we have analyzed the spatial expression patterns of GLUT-1 and CA IX in relation to (i) each other, (ii) the CD34/αSMA-positive tumor microvasculature, (iii) Ki67-positive proliferating cells and (iv) two key proteins widely believed to be causally involved in the “Warburg phenotype” of hypoxia-independent, aerobic glycolysis of malignant cells, hexokinase (HK)-2 [15] and pyruvate kinase (PK)-M2 [16].