In the present study, we validated that the miR-638 expression was significantly decreased in CRC compared with NCT in an expanded CRC cohort, and miR-638 can inhibit CRC cell proliferation, invasion and induce cell cycle arrest in G1 phase by directly targeting TSPAN1, a molecular that appears to be an independent prognostic factor for CRC. The gene discussed is TSPAN1; the disease is colorectal carcinoma.