To further confirm that TSPAN1 is a direct target of miR-638 in CRC, we mutated the predicted binding site of miR-638 in the TSPAN1 3′UTR (Figure 3E), and found that the mutant 3′UTR was completely refractory to miR-638-mediated luciferase reporter repression in both HEK-293T and HCT-116 cells (Figure 3F). This evidence concerns the gene TSPAN1 and colorectal carcinoma.