The purpose of this study was to perform a molecular characterisation of CDCP1 and CD9 protein expression in a panel of colon cancer cell lines and, given the proposed role of CDCP1 in metastasis, to assess the effect of CDCP1 expression on properties of these cancer cells that are directly relevant to metastasis, namely adhesion and motility. Here, CDCP1 is linked to colonic neoplasm.