(TNF-α, 1 h infection; 1.01 ± 0.07, p > 0.05, 6 h infection; 1.27 ± 0.13, p < 0.05, 12 h infection; 1.05 ± 0.05, p > 0.05, 24 h infection; 1.07 ± 0.09 p > 0.05) MyD88 expression increased significantly at 1, 6, and 12 h after M. smegmatis infection. Here, TNF is linked to infection.