Individuals with the GT or TT (GT+TT) genotype at the CYP1B1*2 G355T locus showed a significantly higher risk for developing laryngeal cancer than individuals with the GG genotype (crude OR = 3.141, 95% CI: 2.483–3.974, P <0.001), and this difference between genotype groups remained significant even after adjusting for differences in age, gender, smoking habits, and drinking habits (adjusted OR = 2.657, 95% CI: 2.078–3.398, P <0.001). Here, CYP1B1 is linked to laryngeal carcinoma.