VEGFA and hypothyroidism: Regardless of the experimental group and pregnancy period, VEGF expression in CL was evident mainly in the luteal cells, as its expression was cytoplasmic in all luteal cells (Figure 6A).Hypothyroidism reduced the area and intensity of VEGF immunohistochemical expression at 19 days of gestation compared to controls (P < 0.05) (Figure 6B and 6C), but no significant difference in the gene expression of VEGF in any period of pregnancy was found (p > 0.05) (Figure 7A).