The lack of significant effects of the McN-A-343 infusion on colonic level of IL-4 in mice DNBS-induced colitis observed in our study suggests that IL-4 do not play a role in mediating the beneficial effects of cholinergic modalities on colitis severity, as in the context of colitis IL-4 is known to decrease T cell priming [35]. This evidence concerns the gene IL4 and colitis.