To determine the contribution of CD4+CD25− T cells to the decreased cytokines production observed in the spleen following activation of the CAP during DNBS-induced colitis, we isolated CD4+CD25− T cells from the spleen of naïve animals and determine the splenic CD11c+ DCs priming of CD4+CD25− T cells and their subsequent cytokine release in the presence of anti-CD3/Cd28 Abs. The gene discussed is CD28; the disease is colitis.