Studies examining hepatocellular carcinoma and colon cancer also revealed that the expression of MTSS1 was positively associated with lymph node metastasis (9,10), but the results from the present study demonstrate no statistically significant difference in the MTSS1 expression levels between patients with lymphatic metastasis and those without. This evidence concerns the gene MTSS1 and metastatic malignant neoplasm in the lymph nodes.