In the present study, to clarify the mechanisms involving GRP94 in the pathogenesis of gastric cancer induced by the ER-α36 signaling pathway, SGC7901 cells were treated with E2; increased GRP94 and ER-α36 expression levels, as well as increased phosphorylation levels of Akt at Ser473, were observed. The gene discussed is HSP90B1; the disease is gastric cancer.