For the first set of studies, we isolated CSC enriched CD44+EpCAMhigh cell populations from human CRC cell lines SW480 and HT29 [53] and then subjected them to sphere cluster formation assays (Figure 5) [53] in the absence or direct presence of GSE (50–100μg/mL), and % of floating colonospheres (≥ 50 cells per sphere) generated after 10 days were determined (Figure 5A-B). This evidence concerns the gene CD44 and colorectal carcinoma.