In the present study we (i) profiled a panel of ovarian cancer and normal ovary surface epithelial cell lines for the expression of GBGT1, NAGA, and the other glycogenes encoding the glycosyltransferases and glycosidases involved in the biosynthesis of Fs, (ii) investigated whether GBGT1 expression is regulated through DNA methylation and whether the degree of DNA methylation correlates with GBGT1 expression in cell lines and tissue, and (iii) determined whether GBGT1 expression is an outcome predictor in ovarian cancer. The gene discussed is GBGT1; the disease is ovarian cancer.