These 81 tests generated two significant findings: ALDH1A2 rs7169289 was nominally associated with isolated spina bifida cases in the African American population in continuous (GRR = 0.46 [0.25-0.87], p = 0.017) and dominant (GRR = 0.47 [0.24-0.94], p = 0.033) models. The gene discussed is ALDH1A2; the disease is spina bifida.