To ascertain biological relevance of NKG2D–DAP10 in EMT reprogramming, freshly isolated cell suspensions from 12 primary invasive breast cancer specimens (referred to as BT1 to BT12) were examined by multi-parameter surface flow cytometry for CD45, EpCAM, NKG2D, and the E-cadherin−/N-cadherin+ EMT signature. Here, HCST is linked to invasive breast carcinoma.