HCST and breast cancer: For confirmation of principal findings across diverse tumor lines, we tested NKG2D–DAP10 transfectants of SUM149PT breast cancer [9], A375 melanoma [3], and MDAH-2774 ovarian cancer cells, and, in more detail MCF-10AT premalignant mammary epithelial cells, which were co-transduced with lentiviral constructs for doxycycline (Dox)-inducible NKG2D and DAP10 expression (Figure S2A and S2B).