FUS and amyotrophic lateral sclerosis: Studies with several ALS-linked variants in the laser-induced DNA damage assay discussed earlier have produced conflicting results: R244C, R514S, H517Q, R521C (W. Y. Wang et al., 2013), R521G, and R524S (Mastrocola et al., 2013) were recruited to sites of laser-induced DNA damage in U2OS cells to a similar degree as FUS WT, whereas in A549 cells, recruitment of R521G was reduced relative to FUS WT (Rulten et al., 2013; Figure 3).