In addition, Her-2 protein overexpression was found to be significantly associated with the depth of tumor invasion (P=0.017), lymph node metastasis (P=0.016) and TNM staging (P<0.001), but not with age, gender, tumor location, the degree of differentiation or Lauren classification (P>0.05). This evidence concerns the gene ERBB2 and metastatic malignant neoplasm in the lymph nodes.