Moreover, the hypermethylated breast tumor gene signature identified in TCGA [35] overlapped with our cluster 3, showing both hypermethylation and reduced expression of genes such as ASCL2, CCND2, COL1A2, EPHB1, FABP3, GAS7, IFNGR2, IRAK3, KLK10, POMC, SCGB3A1, SFRP1, SMO, and VCAN (CSPG2). This evidence concerns the gene ASCL2 and breast neoplasm.