In conclusion, this study detected the high expression levels of GOLPH3, p-Akt, p-mTOR, p-4E-BP1 and p-p70S6 in the gastric cancer group strongly which correlated with histological grade, depth of invasion, distant metastasis, and lymph node involvement by clinicopathological variables analysis and immunohistochemistry. Here, GOLPH3 is linked to gastric cancer.