A cell viability assay using small interfering RNAs (siRNAs) specific for the shRNA-targeting candidate genes revealed that ribosomal protein L31 (RPL31), histone cluster 1 H2bd (HIST1H2BD), and ADAM metallopeptidase with thrombospondin type 1 motif 1 (ADAMTS1) were involved in the proliferation of bicalutamide-resistant prostate cancer cells. The gene discussed is H2BC5; the disease is Familial prostate cancer.