CD44 and breast carcinoma: (Fig. 2A) Breast cancer cell lines (MDA-MB-231, MDA-MB-468, MCF7 and T47D) and primary human breast cancer cells were labelled with pre-conjugated with fluorescently antibodies to CD44, CD24 and ESA (stem cell markers), 7-AAD (viability marker) and Hoechst (Cell cycle).