Two significantly downregulated key modulators of the canonical Wnt pathway GSK3B (-5.28 fold) and CTNNB1 (-3.25 fold) were further verified by qPCR in CLDN3-transfected HepG2 and MHCC97H (a well-known metastatic HCC cell line), as well as in CLDN3-knockdown Huh6 cells (Fig. 6B). The gene discussed is CLDN3; the disease is hepatocellular carcinoma.