Using qPCR analyses, we found the metastasis suppressor role of CLDN3 in HCC cells was associated with the inhibition of Wnt-EMT activity through downregulation of GSK3B (encodes GSK3β), CTNNB1 (encodes β-catenin), SNAI2 (encodes Slug), and CDH2 (encodes N-cadherin) by qPCR analyses. This evidence concerns the gene GSK3B and hepatocellular carcinoma.