Stable TAK1-overexpressing clones were generated by transfection of TAK1 plasmids into ovarian cancer cell lines; OVCA429 (429-C12 and 429-C13) and A2780cp (Acp-T2 and Acp-T3), while stable knockdown of endogenous TAK1 was achieved in SKOV3 (SK-sh1-KD3 and SK-sh1-KD6) and A2780cp (Acp-sh1-KD1 and Acp-sh2-KD10) cells using vector-based RNAi constructs (Figure 2A-B). Here, NDUFAB1 is linked to ovarian cancer.