In order to understand the mechanism by which MDM2-mediated p53 degradation is prevented in RCC cells upon TRIM8 over-expression, we performed co-immunoprecipitation experiments, which demonstrated that MDM2-p53 binding is easily displaced when TRIM8 is expressed (Figure 5), as we previously found in HCT116 cells [22]. Here, MDM2 is linked to renal cell carcinoma.