In the present study, the cytotoxicity of a group of novel 1,3-diphenylprop-2-en-1-one chalcones are determined on three COX-2 expressing cell lines; human breast MCF-7 adenocarcinoma (16), human lung A549 adenocarcinoma (17), and human HepG2 hepatocarcinoma (18). This evidence concerns the gene PTGS2 and adenocarcinoma.