However, compared with those by CLP-induced ALI, the mRNA and protein expression levels of Tollip were significantly increased with the administration of XBJ, and TLR4, TRAF6, p-IRAK1 and NF-κB gene and protein expression was significantly decreased (P<0.05; Figs. 1–4). The gene discussed is TLR4; the disease is acute respiratory distress syndrome.