HMGA1 and breast adenocarcinoma: To evaluate the ability of these miRNAs to target HMGA1P6 and HMGA1P7, we transfected miR-15, miR-16, miR-214 and miR-761 into MCF7 cells (human breast adenocarcinoma), and measured HMGA1P6, HMGA1P7, and HMGA1 mRNA levels using Real-time PCR and PCR primer sets (see Methods) that discriminate the three mRNA transcripts.