VEGFA and Hyperglycemia: VEGF significantly decreased the neurite outgrowth (axonal length μm ctrl 93.8±2.7; VEGF 10 pg/ml 94.5±5.2; VEGF 100 pg/ml 81.7±5.2 p<0.05 vs ctrl; VEGF 1000 pg/ml 83.9±4.2 p<0.05 vs ctrl; VEGF 10 ng/ml 83.3±4.7 p<0.05 vs ctrl; VEGF 50 ng/ml 81.6±3.1 p<0.05 vs ctrl; VEGF 100 ng/ml 72.9±4.7 p<0.05 vs ctrl; Fig. 5E), suggesting that VEGF had a key role in the decrease of neurite length found in DRG co-cultures exposed to hyperglycaemia.