In conclusion, using two glioma cell lines, our data have shown the following: 1) MAZ51 causes dramatic shape changes due to the clustering and aggregation of F-actin and microtubules, 2) MAZ51 induces cell cycle arrest at the G2/M phase in rat glioma C6 and human glioma U251MG cell lines, 3) MAZ51 selectively targets transformed cells but not primary astrocytes, 4) Akt-mediated inhibition of GSK3β and activation of Rho are involved in the effects of MAZ51, and 5) the effects of MAZ51 are independent of the inhibition of VEGFR-3 phosphorylation in rat glioma C6 cells. The gene discussed is AKT1; the disease is central nervous system cancer.