To test if WFA when used alone or in combination with CIS targets CSCs, we performed immunohistochemical analysis of the tumors collected from the mock-treated animals and animals treated with WFA and CIS both alone and in combination using the antibodies for markers expressed by putative CSCs including CD44, CD24, CD34, CD117 and Oct4 [56]. This evidence concerns the gene CD34 and in situ carcinoma.