In limb buds, Twist1 binding to peak sequences associated with wingless-related MMTV integration site 5B (Wnt5b), lymphoid enhancer binding factor 1 (Lef1), nemo like kinase (Nlk), casein kinase 1, epsilon (Csnk1e), disabled 2 interacting protein (Dab2ip), mutated in colorectal cancers (Mcc), and frizzled homolog 3 (Fzd3) were confirmed (Figure 6B). The gene discussed is LEF1; the disease is colorectal cancer.