The correlation between the levels of expression of these candidate biomarkers and the clinicopathological features of patients included in the study, indicated that the expression of BRAF, PIK3CA, RELA, RUNX1 (all involved in endometrial carcinogenesis) and CD44 (stem cell marker) was increased in CTC isolated from patients with the tumor infiltrating more than 50% of the myometrium (n = 18) compared with those isolated from patients having myometrial invasion below 50% of the myometrium (n = 13) (Additional file3). This evidence concerns the gene PIK3CA and neoplasm.