Finally, in order to determine whether epigenetic changes influence spinophilin expression in CRC, we treated the HCT116, RKO, Colo320 and Colo201 cell lines with the de-methylation agent 5-aza-dC and the histone deacetylase inhibitor trichostatin A(TSA). This evidence concerns the gene PPP1R9B and colorectal carcinoma.