To look into the influence of high glucose (HG) levels on resistance to chemotherapeutic agents, several human cancer cells, carrying wild-type (wt) p53, including RKO and HCT116 colon cancer, 2008 ovarian cancer and A549 lung cancer cells, were incubated with chemotherapeutic drugs, such as adryamicin (ADR) or cisplatin (CDDP), before or after treatment with HG (4.5 g/L D-glucose) condition. Here, TP53 is linked to colonic neoplasm.