A total of 27 DEGs were significantly enriched in 16 KEGG pathways, such as protein digestion and absorption, fatty acid metabolism, amoebiasis, etc. Furthermore, the regulatory network of these 27 DEGs was constructed, which involved several key genes, including protein tyrosine kinase 2 (PTK2), v-src avian sarcoma (SRC) and Caveolin 2 (CAV2).<h4>Conclusion</h4>PTK2, SRC and CAV2 may be potential markers for diagnosis and treatment of ALI.<h4>Virtual slides</h4>The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/5865162912987143. The gene discussed is PTK2; the disease is acute respiratory distress syndrome.