Conversely, DNA demethylation obtained through 5AZA treatment reproduced in vitro the downregulation of the transcript observed among cell lines and cancer biopsies in vivo. In fact, in treated cells, the levels of the B3GALT5 LTR transcript decreased from 3–10 to less than 0.2 fg/pg β-actin, while the amounts of HNF1 remained unchanged, as found in colon cancer biopsies. This evidence concerns the gene HNF1A and malignant colon neoplasm.