Figs. 4A to C show the results of Western blotting and densitometry analysis of the blots. The phosphorylation levels increased significantly in WT hearts after TAC except for CaMKI; however, the phosphorylation levels of AMPK, CaMKI, and CaMKIV were significantly reduced in α-MHC CaMKKβkd TG hearts compared with the WT mice after TAC. The gene discussed is PRKAA2; the disease is persistent truncus arteriosus.