To further support the hypothesis that out-of-sequence signal 3 CD8 T cells do not receive the positive effects that type 1 IFN can have as a signal 3 cytokine during acute virus infection, and thereby contribute to suppression of proliferation, we determined the frequency of poly(I∶C)- or HBSS-pretreated P14 cells after cognate peptide stimulation. The gene discussed is CD8A; the disease is viral infectious disease.