As can be seen in Figure 6A, the γH2AX stained area, which is elicited by S139 phosphorylation of histone H2AX by DNA damage, was clearly observed within the pimonidazole-positive hypoxic area 24 h after pyruvate/TH-302 administration to SCCVII tumor-bearing mice on day 7. The gene discussed is H2AX; the disease is neoplasm.