It was considered that the measuring of free testosterone (FT) by equilibrium dialysis either calculated free testosterone (cFT), calculated bioavailable testosterone (cBT) from total testosterone (TT), sex hormone–binding globulin (SHBG) and albumin, or the free androgen index (FAI), were more sensitive methods for assessment hyperandrogenemia in women with PCOS and hirsutism (8, 9). The gene discussed is SHBG; the disease is Hirsutism.