GCLC and cancer: GCLC protein was increased in the GI tract of wild type mice only, but not liver; whereas in both normal (RL-34) and cancer (Hepa-1c1c7) murine cell lines, all three (NQO-1, GST, and GCLC) proteins were increased in response to an aqueous extract of broccoli seed in wild type, but not in Nrf2−/− cell lines [82].