CD8A and neoplasm: We engineered H2-Kb B16 tumors to differentially express a model tumor antigen (influenza HA H2-Kd restricted CL4 epitope expressed in frame with EGFP) in the secretory, cytoplasmic, or nuclear compartments, and compared their potential to induce proliferation of H-2Kd restricted CL4-specific CD8+ T cells in vivo as a measure of antigen cross presentation.