CD8A and neoplasm: To study the effect of cellular localization on tumor-specific CD8+ T cell responses, we modified B16 tumor cells to express the MHC class I Kd restricted immunodominant epitope of the influenza virus HA protein, CL4, fused with EGFP, as a quantifiable model tumor antigen in nuclear (B16.Nuc), secretory (B16.Sec), or cytoplasmic (B16.Cyto) compartments (Fig. 1A).