Equal amounts of proteins were used for SDS-PAGE, followed by western blotting and detection with anticaveolin-1 and antiflotillin-1 antibodies and anti-β-actin for normalization; in most cases caveolin-1 and flotillin-1 bands resulted more intensely in HCC tissue samples than in autologous ANT (Figure 3(a)). This evidence concerns the gene FLOT1 and hepatocellular carcinoma.