Tumour cells were negative for leukocyte common antigen (LCA), S-100, WT1, desmin, myogenin, neuron specific enolase (NSE), CD34, CK7, and TTF-1 excluding lymphoma, melanoma, Wilms' tumor, rhabdomyosarcoma, neuroblastoma, epithelioid sarcoma, and rhabdoid large cell carcinoma of lung, respectively. The gene discussed is ENO2; the disease is melanoma.