S100A4 and neoplasm: Compared with the primary tumor, CTC-c cells demonstrated clear loss of the epithelial markers E-cadherin (Cdh1) and Muc1 (Figure 3B), whereas mesenchymal transcripts were mixed, with some showing increased expression (Cdh11, Vim) and others with reduced levels (S100a4, Itga5, Sdc1) (Figures 3C and 3D).