To minimize the possibility that any effects could be due to “off-target’ silencing, we performed reconstitutions with two independent Brd4 shRNAs (shBrd4.1448 and shBrd4.552) (Figure S1A), previously shown to potently inhibit Brd4 expression in AML cells (Zuber et al., 2011b). The gene discussed is BRD4; the disease is acute myeloid leukemia.