To understanding whether ADI down-regulated the phosphorylation of NF-κB p65 by blocking activation of the PI3K/Akt survival signal pathway, we evaluated the expression of several important proteins in this signaling pathway in pancreatic cancer cells treated with ADI in combination with the PI3K inhibitor LY294002 treatment for 20 min. Here, AKT1 is linked to familial pancreatic carcinoma.