HIF1A and ischemia: As shown in Figures 4A and 4B, post-ischemia treatment with Ast IV significantly increased HIF-1α, iNOS, and Bcl2 protein expression, to 4.36±0.20-fold, 4.11±0.14-fold, and 4.14±0.17-fold of the control, respectively, whereas Caspase3 expression decreased to 0.45±0.087-fold that of the control (vs. SIR group, P<0.01).