Furthermore, prophylactically PACAP-treated mice displayed higher numbers of ileal FOXP3+ Tregs as compared to control mice at day 7 p.i. In support of this, PACAP−/− mice exhibited reduced numbers of proliferating Tregs in experimental autoimmune encephalomyelitis [38], [39]. This evidence concerns the gene FOXP3 and experimental autoimmune encephalomyelitis.