Cases of Hb E trait, Hb E-β-thalassemia, and sickle cell-E disease showed moderate to severe anemia, and target cells, and reduced values of red cell indices like red blood cell count, Hb level, hematocrit, mean cell volume, mean cell Hb and mean cell Hb cencentration, describing abnormal hematological profile and clinical manifestations before blood transfusion. This evidence concerns the gene GSTM1 and anemia (phenotype).