The objective of this work was to describe and develop HPTLC analysis (fingerprint and densitometry) for the determination of flavonoids in plant extracts (Asparagus racemosus, Withania somnifera, Vitex negundo, Plumbago zeylanica, Butea monosperma and Tephrosia purpurea), and to determine the validity of plant remedies used for gout by examining their antioxidant and xanthine oxidase inhibitory activity. The gene discussed is XDH; the disease is gout.